eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-01
12
1
1
20
10.21608/ejbbe.2011.1183
1183
Original Article
Effect of Laser Beam on Microorganisms Resistive to Carcinoma
MICROBIAL THERAPY is being used to control the tumor growth. However the control of the activity of the microbe faces several problems which limited its applicability. Therefore, the aim of the present study was to assess the possibility of controlling the activity of Pseudomonas aeruginosa by 650 nm diode laser beam; power density 150 mW/cm2, the spot area of the laser beam was 1 cm2, incident doses of 30 joules/cm2 was applied for 30 min per day. The tested mice were divided into 7 groups A, B, C, D, E, F and G. Group A was used as control, group B injected in the right thigh by 0.2 ml from a suspension containing 106 cell /ml of the Ehrlich tumor, group C injected with 4x109 CFU of Pseudomonas aeruginosa. group D injected in the right thigh by 0.2 ml from a suspension containing 106 cells/ml of the Ehrlich tumor and exposed to Laser radiation for 30 min /day along four successive days. The group E injected with 4x109 CFU of Pseudomonas aeruginosa, and exposed to Laser radiation for 30 min /day along four successive days. The group F mice injected in the right thigh by both 0.2 ml from a suspension containing 106 cell/ ml of the Ehrlich tumor and 4x109 CFU of Pseudomonas aeruginosa. The group G injected in the right thigh by both 0.2 ml from a suspension containing 106 cell/ml of the Ehrlich tumor and 4x109 CFU of Pseudomonas aeruginosa and exposed to Laser radiation for 30 mins/day along four successive days. The tumor growth characteristics were followed for all animals. Cellular changes were evaluated using cells viability (ATP production) and cytokines expression (IFN- γ and IL-6) the treated and untreated tumors were studied and survival rate were demonstrated. The results showed that injection with the microorganism alone caused the death of all animals till fourth day. While, the mice with post tumor implantation (PI) which exposed to laser irradiation decreased the volume of the tumor. On the other hand, tumors infected with microorganisms then irradiated for 1 day post infection showed a sudden decrease in tumor volume during next three days of infection then the tumor began to grow again with higher rate. More than 70% of the animals survive till day 35 PI. The groups A, B, C and F in the 1st, 2nd, 3rd and 4th days produced a non significant increase in ATP luminescence. While, the groups D, E and G irradiated once at the 1st, 2nd, 3rd and 4th day produced a significant increase in ATP. In addition, the groups A, B, C and F cells showed a significant increase in the level production of IFN-γ and IL-6 in serum. Moreover, Irradiated groups D, E and G produced a significant change of both cytokines, but, the group G exhibited a highly significant increase in the production of IFN-γ and IL-6 in serum more than the groups D and E.
https://ejbbe.journals.ekb.eg/article_1183_1eec4da4b3c0173bee49ca021457af10.pdf
Diode laser
Microbial therapy
Pseudomonas aeruginosa
Ehrlich tumor
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-31
12
1
21
31
10.21608/ejbbe.2011.1184
1184
Original Article
Role of Some Fatty Acids in the Dynamics of Follicular Fluid in She- Camel
THE MAIN objective of this work was to determine the activity of some …..antioxidants and content of fatty acid in the follicular fluid in relation to size of the follicle and quality of oocyte in she camels (Camelus dromedaries). The vesicular follicles (≥ 3mm in diameter) were selected from the obtained ovaries, and classified according to their surface diameter into small, medium, large and very large size follicles (3-5, > 5-10, > 10-15 and > 15mm, respectively). The aspirated follicular fluid was centrifuged to separate cell fraction and then examined for oocyte quality. Most of the recovered small and medium size follicles were of good quality (69.23 and 59.19%, respectively) in comparison to those recovered from large and very large ones (33.33 and 23.08% , respectively ). The activity of SOD (superoxide dismutase) was elevated and those of GSH (glutathione) dropped in the follicular fluid as the diameter of follicles increased and the percentage of oocytes with good quality was decreased. Palmitic (C16:0) and oleic (C18:1) acids were the most abundant fatty acids in the follicular fluid, while, those of stearic (C18:0), linoleic (C18:2) and arachidonic (C20:4) acids were lower. Other fatty acids were very low. Moreover, the content of stearic (C18:0), oleic (C18:1), linoleum (C18:2) and arachidonic (C20:4) acids in the follicular fluid declined as the diameter of follicles was increased and the percentage of oocytes with good quality decreased. Those of other fatty acids did not vary significantly with the change in the size of follicle and /or quality of oocytes.
https://ejbbe.journals.ekb.eg/article_1184_aad98ce71044b6684ee9026d24c5dee1.pdf
follicular fluid
oocytes
fatty acids
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-31
12
1
33
44
10.21608/ejbbe.2011.1185
1185
Original Article
Monitoring of Omega -3 level as a Marker of Oxidative Stress in Rats Exposed to Microwave Radiation
THIS STUDY aimed to investigate firstly, the oxidative stress and …..erythrocyte hemolysis due to the effect of exposure to 2450 MHz continuous microwave (MW), which is the carrier of signals emitted by cellular phones. Secondly to evaluate the possible protective role of omega–3 supplementation. Before exposure to microwaves, male rats were randomly divided into four groups, the first group was considered as control, the second group was exposed to microwave at frequency 2450 MHz alone, the third group was supplemented by omega -3 (40 mg/kg) and the fourth group was exposed to MW but supplemented by omega -3 (40 mg/kg) before irradiation. The level of Malondialdehyde (MDA), an index of lipid peroxidation (LPO), was estimated and used as a marker of oxidative stress. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were expressed to evaluate the changes of antioxidant status. Also erythrocyte osmofragility test was assessed to determine rate by which red blood cells will undergo hemolysis. The level of malondialdehyde significantly elevated and the levels of some antioxidative enzymes significantly decreased, in addition the erythrocyte hemolysis rate increased in the MW group (second group) as compared to the control group. Oral administration of omega -3 (OT) exhibited marked amelioration of LPO, antioxidative enzymes, and rate of hemolysis which returned to normal values during the course of OT treatment. It could be concluded that OT has an obvious antioxidant effect that may be able to compensate MW-induced oxidative changes in the blood tissue by enhancing the antioxidant defense system.
https://ejbbe.journals.ekb.eg/article_1185_628dff9f50d13f6727133f3a8c1730ea.pdf
microwave
omega-3
antioxidant
Erythrocyte
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-31
12
1
45
55
10.21608/ejbbe.2011.1186
1186
Original Article
Influences of Architectural Shapes of Environment on Electrical Activity of Rat's Brain
THE ARCHITECTURAL shapes of environment may affect the activity of living inhabitants. Accordingly the present work was aimed to study and clarify the influences of architectural shapes of environment on the electrical activity of rat's brain. The brain activities (electroencephalogram, EEG) recorded from rats housed into 2 different models, pyramidal and inverted pyramidal shapes for 3 weeks were studied. The obtained results showed a drop in the mean power amplitude of almost all frequency bands of rat's brain activities placed in pyramidal shape especially of θ , α and β bands. This effect was more pronounced in motor than in visual cortex. On the other hand the recorded rat's brain activities housed in an inverted pyramidal shape showed an enhancement in the mean power amplitude of all frequency bands. The obtained results proved that rats kept in pyramidal shape were more relaxed and more active than that inside an inverted pyramidal environment.
https://ejbbe.journals.ekb.eg/article_1186_6d79687388b488059091c6001ba8bdd8.pdf
EEG
Architectural shape
Pyramidal
Inverted pyramidal
environment
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-31
12
1
57
67
10.21608/ejbbe.2011.1187
1187
Original Article
Effects of High Energy Electron Beams on Small Fields by Using TPS
ENERGIES of electron beams studied were 6, 8, 10, and 15 MeV. ……Small fields from 2x2 to 4x4 cm2 were obtained from Cerrobend shields (cutout) attached to the bottom face of a 6 x 6 cm2 electron cone applicator. Measurements were carried out in a solid phantom with a Roos ionization chamber and Kodak X-OmatV films.
For small field this extra shielding will affect the percentage depth dose (PDD) and the output factors due to little of lateral scatter. The change in dose at maximum depth (d
max) as well as changes in the PDDs with small field must be accounted for when measuring output factors.
The aim of the present work was to achieve an accurate calculation of dose for small field dimensions and performing this by evaluating the accuracy of planning system calculation. This will be compared with real measurement of dose for same small field dimensions using different detectors.
Practical work was performed in two steps: (1) Determination of same physical factors required for dose estimation measured by Ross ionization chamber and calculated by treatment planning system (TPS) based on the latest technical report series (IAEA TRS-398). (2) Comparison of calculated and measured data was done.
For all values energies of used where the field area of the electron beam becomes smaller this lead to the following: (i) The PDD of maximum dose shifts toward the surface, (ii) The depth of 90% and 80%, which are common dose for electron energy, becomes smaller, (iii) The surface dose increases due to scattering and (iv) the dose fall-off region becomes more gradual.
The output of the electron beam decreases significantly with reduction in electron beam field area as a consequence of mild lateral equilibrium.
It can be concluded by considering the milde difference between the calculated data and measured values by Roos IC from 3x3 to 10x10 cm
2 field dimenions, where as for 2x2 cm2 field dimenion this difference exceeded 40% for all energy. Radiographic film showed a variation in results according the following: 10% for 6 MeV, less than
https://ejbbe.journals.ekb.eg/article_1187_fcb75c73bbe5668c2e015fc89002126c.pdf
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
2011-12-31
12
1
69
85
10.21608/ejbbe.2011.1188
1188
Original Article
Effect of Mg2+ Doping on Formation of Apatite Layer by Bioactive Sol-Gel Glass-Ceramic
BIOACTIVE glass–ceramics of mixed alkali and alkali earth were ……prepared by quick alkali mediated sol–gel method. The influence of varying MgO content substituted for SiO2 on the structure and thermal behavior of glass compositions in the SiO2–Na2O–CaO–P2O5–MgO system. This system was investigated by DSC, TGA, XRD and FTIR. In addition, to study in-vitro bioactivity of these glass-ceramics were characterized by analysis of apatite-formation layer in simulated body fluid (SBF). The obtained results revealed that at sintering temperature above 900oC, crystallization occurred and glass-ceramics which contain wollastonite, diopside and Calcium phosphate silicate. An increase in concentration of MgO at the expense of SiO2 led to the formation of diopside in the glass-ceramic. The present results of in vitro tests showed that tendency of apatite layer formation decreased with increasing quantity of diopside phase.
https://ejbbe.journals.ekb.eg/article_1188_add50627a19a4c69c39743de90f0389e.pdf
sol-gel method
Bioactive glass-ceramic
Diopside
In-vitro test