Molecular Structure Changes Of Rat’s Retina After Sodium Iodate Injection and The Effect Of Taurine

Document Type : Original Article

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1 1,3 Physics Department, Biophysics Branch, Faculty of Science, Al-Azhar University (Boys)

2 Biophysics&Laser Science Unit, Research Institute of Ophthalmology,

3 Physics Department, Biophysics Branch, Faculty of Science, Al-Azhar University (Boys), Cairo

Abstract

The aim of present work is to investigate the mechanism of action of sodium iodate (NaIO3) on the molecular structure of retina and whether the nutritional intake of taurine may delay the development of age macular degeneration (AMD) disease induced by NaIO3 using Fourier transform infrared spectroscopy (FTIR). One hundred and five rats (200-230 g) are classified into three groups. I (15 rats) used as control, II (45 rats) received a single intravenous injection of 25, 50, or 75 mg/kg NaIO3, and III (45 rats) supplemented with 4 g taurine /100 g diet for two weeks before injection of NaIO3. The injection continued until decapitation. All injected doses of NaIO3 are associated with vibrational changes that include decrement of the modes of vibration related to stretching OH and amide II, significant increases (p˂0.05) in wavenumber and width of asymmetrical OH and disappear of asymmetrical NH3. Also, there are significant diminish (p˂0.05) in α-helix content in the same context, in addition to significant increments (p˂0.05) in β-Sheet indicating the conformational changes of the secondary structure of protein due to NaIO3 injection. Enhancement of most changes that previously detected in FTIR spectra are noticed after taurine treatment especially before and after low dose of NaIO3. NaIO3 is considered to be specific for animal model as age macular degeneration. Taurine action is efficient as antioxidant to protect retina from oxidative stress limits in range of 25 mg of NaIO3 and decreases the efficiency by increasing the dose of NaIO3 up to 75 mg.

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