National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Dose Verification with diode versus TLD as in-vivo dosimeters for external radiotherapy
1
9
EN
Adel
Elsayed
Yassin
Oncology Department Faculty of Medicine Al-azhar university Cairo Egypt
a.yassin79@gmail.com
khaled
el shahat
Radiation Oncology Department, Faculty of medicine, Al-Azhar University
khelshahat@yahoo.com
Ehab
Marouf
Attalla
Radiotherapy & Nuclear Medicine Dep.; National Cancer Institute ; Cairo university
attalla.ehab@gmail.com
Hassn
Khaled
Radiation Oncology Department, Faculty of medicine, Al-Azhar University
hassankhalid_57@yahoo.com
10.21608/ejbbe.2020.22441.1029
Background: Conformal radiotherapy was the first step toward patient-specific treatment, this is, in turn, arise the need to perform quality dose verification (DV) with in-vivo (IV) dosimetry. Aim: two common IV dosimeters were compared separately, diode and thermoluminescent dosimeters (TLD), the dose was compared with calculated dose and the isocenter dose measured with ion chamber (IC). Materials and Methods: First The absolute dose was measured in PTW Farmer IC (0.6 cc) on Varian machine model Unique for twenty cancer patients planned in 3D conformal radiotherapy, they were randomly selected to cover different clinical sites, the dose was calculated in Eclipse treatment planning system (TPS). Then in the treatment position, the in-vivo DV dosimetry was performed using: Sun nuclear Semiconductor diode and TLD. Results: the dose measured with Diode had less variance (-1.94±2.05) with TPS than dose measured with TLD/TPS (4.08 ±6.51), when comparing the dose measures with IC this discrepancy is not the case with IC/TPS as they were measured in the isocentre point and compared with prescribed doses Conclusion: patient specific DV performed with IC is the most accurate as a double check with prescribed dose. For IV dosimetry diode was closer to calculated dose, however, both diode and TLD characterized by uniform difference versus calculated dose within different clinical sites which keep each of them an accepted device especially when seeking their additive values either of small field dosimetry with TLD or skin dose measurement with diode. Those outcomes highlight the limitation of each device for IV dosimetry.
Dosimetry,dose verification,Treatment planning system (TPS),Diode,TLD
https://ejbbe.journals.ekb.eg/article_82526.html
https://ejbbe.journals.ekb.eg/article_82526_aee81cbb43308c3db1298a74ceb435ed.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
The Protective role of Moringa oleifera against effects of electromagnetic field on Blood of Albino rats
11
17
EN
Shoman
H
M
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
dr.hodashoeman@hotmail.com
Aida
A
Salama
Biophysics Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
salama.aida@yahoo.com
Aziza
A
Elsaeid
Biophysics Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
azelsaeid@yahoo.com
Ola
Mohamed
Awad
Biophysics Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ola_adel14762@yahoo.com
10.21608/ejbbe.2020.26864.1030
Abstract: The purpose of this study was to explore the capability of Moringa oleifera leaf extract in protecting rat blood against the exposure to electromagnetic field (EMF). Forty two adult white Albino male rats, weighting (150-180 g) were divided into six equal groups . The first group is the control while the second group was orally supplemented daily with (200 mg/kg) of Moringa oleifera leaves extract (MOLE) for six days. The third group was continuous exposed to EMF of 1.5mT, 50 Hz for 9 hours. The other three groups were exposed to the same EMF and Moringa extract in different ways (before, after and before and after the exposure of magnetic field). Blood samples were collected and hemoglobin extracted. The relative viscosity and conductivity were measured. Blood films were also taken for histological examination. The results showed significant increase in relative viscosity and conductivity of hemoglobin molecules. Microscopic examination of blood films showed alterations in different shapes and pale in color of red blood cells with aggregation to form rouleaux. Lymphocytes degeneration were also observed. The results of groups which treated with Moringa showed good repairing in all results. So this study concludes that impairments caused by EMF on blood can be decreased by Moringa oleifera leaf extract.
Electromagnetic Field,Moringa oleifera,Blood
https://ejbbe.journals.ekb.eg/article_109434.html
https://ejbbe.journals.ekb.eg/article_109434_de8de2d47a199421f10291ec2dfa9866.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Preparation, characterization and evaluation of cytotoxic activity of Tamoxifen bound liposomes against breast cancer cell line
19
31
EN
Medhat
Shafaa
0000-0003-1807-6516
Physics Dept., Medical Biophysics Division, Faculty of Science, Helwan University
medhatwi@hotmail.com
10.21608/ejbbe.2020.29656.1032
The goal of this study was to determine whether neutrally or positively charged liposomes encapsulated with tamoxifen could inhibit the proliferation of human breast cancer MCF-7 cell proliferation compared to free tamoxifen by estimating the potential effects of these complexes on the cell death of human breast carcinoma (MCF-7) cell lines. The formulated tamoxifen liposomal conjugate was characterized by analytical techniques to determine its size, size-distribution, thermotropic changes and conformational changes along with possible cytotoxicity towards MCF-7 (breast cancer cell line) in Vitro. The mean particle diameter was estimated to be 64.70±49.75 nm, 73.72±23.8 nm and 119±54.7 nm for blank liposomes, neutral and positively charged liposomes bound tamoxifen, respectively. As tamoxifen is introduced into positive liposomes, it results in noticeable broadening and shift to lower temperature at 135 C compared to the main characteristic endothermic peak (Tm) of pure liposomes that exists at 140C, while the introduction of tamoxifen into neutral liposomes contributes to the disappearance of the main endothermic peak of pure liposomes. FTIR analysis revealed structure changes in vesicles after tamoxifen was incorporated into liposomes. The IC50 value for tamoxifen in the cytotoxic assay with MCF-7 treated cells was 0.34 μg/ml, while tamoxifen-loaded neutrally or positively charged liposomes showed an increase of IC50 value to 119.62 and 101.23 µg /ml, respectively. Current data suggests a new treatment regimen in where free tamoxifen is substituted with liposomal tamoxifen to improve anticancer activity against MCF-7 cancer cell lines.
tamoxifen,charged Liposomes,DSC,FTIR,Cytotoxicity
https://ejbbe.journals.ekb.eg/article_109435.html
https://ejbbe.journals.ekb.eg/article_109435_04ae89755b0e2771a8926223e55d559b.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Silica Nanoparticles as a Potential Carrier for Doxycycline Hyclate
33
41
EN
Mohamed
M
Ashour
0000-0002-4295-4975
Basic Sciences Department, Badr University in Cairo (BUC)
mohamedashour739@gmail.com
Islam
Soliman
0000-0003-2934-1231
Department of physics, faculty of Science, Al Azhar University, Cairo, Egypt.
islam_biophysics@yahoo.com
Mostafa
Mabrouk
Refractories, Ceramics and Building materials Department, (Biomaterials group), National Research Centre, 33El Bohouth st.(former EL Tahrir st.)- Dokki- Giza- Egypt
mostafamabrouk.nrc@gmail.com
Hanan
H. Behere
Refractories, Ceramics and Building materials Department, National Research Centre
hananh.beherei@gmail.com
Khairy
Tohamy
0000-0002-1655-5911
physics department,Faculty of Science, Al-Azhar University, Cairo, Egypt
already_a555@yahoo.com
10.21608/ejbbe.2020.40105.1034
Abstract: Silica nanoparticles (SiO2 NPs) have found applications in many advanced biomedical fields. This research work is concerned with the preparation of amorphous silica nanoparticles by the hydrolysis of tetraethyl orthosilicate (TEOS) in ethanol medium and different surfactants such as cetyltrimethylammonium bromide (CTAB) and polyvinylpyrrolidone (PVP) and study the final properties of the prepared silica nanoparticles. The synthesized nanoparticles were systematically characterized by XRD, FTIR, particle size analysis and TEM. The XRD results revealed the crystallinity of silica nanoparticles. FTIR spectroscopy confirmed the presence of Si-O in the sample. The data obtained from DLS showed that particles with size of 198 nm had prepared. Moreover, the cytotoxicity of blanked silica NPs and Doxycycline Hyclate (DOX) loaded silica NPs on Human bone osteosarcoma cells (Saos-2) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results approved that the fabricated Silica nano particles are biocompatible and have the potential to be a convenient drug carrier.
Silica nanoparticles,Microemulsion synthesis,Cytotoxicity,Doxycycline Hyclate
https://ejbbe.journals.ekb.eg/article_110279.html
https://ejbbe.journals.ekb.eg/article_110279_3177f61918ec85a4aa5bc102df27eece.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
The Role of Texture Analysis and Radiomics in Prediction of Bone Marrow Infiltration in Patients with Lymphoma
43
55
EN
Mahmoud
A.
Kenawy
0000-0002-7512-9242
Nasr City, Cairo, Egypt
mahmoudbiophysics@yahoo.com
Magdy
Khalil
0000-0003-2087-5229
Department of Physics,
Faculty of Science,
Helwan University,
Ain Helwan
11795, Cairo, Egypt
magdy_khalil@hotmail.com
Mahmoud
H.
Abdelgawad
0000-0002-1905-9040
Al-Azhar university, faculty of science, physics department, biophysics branch
mh_abutaha@yahoo.com
H.
H.
El-Bahnasawy
Department of Physics, Faculty of Science, Azhar University, Nasr City 11884, Cairo, Egypt.
bahnasawy1@gmail.com
10.21608/ejbbe.2020.42006.1038
Purpose. To explore the ability of image-based parameters with texture parameters in the differentiation of bone marrow infiltration by positron emission tomography-computed tomography (PET/CT).<br /> Procedures. We retrospectively evaluated the baseline PET/CT scan of 44 patients with histologically proven lymphoma. Seventy-three of features were extracted using CGITA software and statistical analysis were carried out on SPSS program. <br /> Results. Spearman correlation analysis revealed a strong positive correlation between conventional PET metrics and texture features (19 significant association features) and inverse correlation was found (one parameter significant association feature). Area under the curve and p-value of receive operating characteristics showed that (HILRE (4-bin), HILZE (64-bin), LRE (64-bin), LZE (64-bin), max spectrum (8-bin), busyness (64-bin),code similarity (32-bin & 64-bin)) were significant discriminator of bone marrow infiltration among other features (AUC>0.682, p < 0.05). Univariate analyses of texture features showed that code similarity and LRE (both 64 bin) has significant prediction to BMI. Multivariate analyses revealed that LRE (64 bin) p= 0.031; odds ratio: 1.022; 95% CI, 1.002-1.043) were independent variables for bone marrow infiltration.<br /> Conclusions. Significant associations emerged between PET features and bone marrow infiltration in lymphoma. Texture analysis on PET/CT shows potential to differentiate between bone marrow infiltration in patients with Lymphoma.
Keywords: Lymphoma,18F-FDG PET/CT,Texture analysis,Bone marrow,Radiomics
https://ejbbe.journals.ekb.eg/article_118528.html
https://ejbbe.journals.ekb.eg/article_118528_2874a4075847210a620dc5ae67f27712.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Effect of low dose He-Ne laser on stored human Blood
57
64
EN
Moustafa
I O
Ibrahim
Egypt, Benha University, faculty of science, physics, biophysics
mustafa.ibrahim@fsc.bu.edu.eg
10.21608/ejbbe.2020.2551.1011
The aim of the present work is to study the effect of He-Ne laser (632.8 nm, 2 mm spot diameter, 30 mW, and continuous pulse) on the properties of human blood during different storage periods and seeking for the optimum laser dose to enhance blood shelf life. Human blood from five healthy donors was collected in human blood bags under the supervision of the center blood bank. The control and treated samples were stored in center blood bank refrigerator at 2-60C. The collected blood was divided into 4 groups, one for the control sample and the three other groups for the irradiation with different doses of He-Ne laser (0.0287, 0.0563 and 0.198 J/cm2 ). Each group was sub-divided into 6 samples to perform during storage days (9, 24, 30, 35, 42, & 50). The measurements of the fragility (Ϝ) and viscosity (η) values of all samples during the storage days suggested that the He-Ne laser at a dose 0.198 J/cm2 is working to increase the resistance of the membrane of the red blood cells (RBC) and consequently the storage period increases form 35 day to 42 days. In addition, viscosity for human blood before and after exposure to He-Ne laser dose of 0.198 J/cm2 reflects the validity of the blood during the storage period. The obtained data proved that the ability of using the recommended He-Ne laser dose 0.198 J/cm2 to improve the rheological properties and prolong the storage period of human blood from 35 days to 42 days.
He-Ne laser,human blood,fragility,viscosity,Storage period
https://ejbbe.journals.ekb.eg/article_122194.html
https://ejbbe.journals.ekb.eg/article_122194_3de16bd684c3ee37e49c22e9dcb7b649.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
physical properties of (SiO2-CaCl2) doped with 3-methacryloxypropyl trimethoxysilane (MAPTMS)
65
73
EN
Ahmed
Ali
Abdel-Aal
physics department, faculty of science, Al-Azhar University, Cairo, Egypt.
ahmed.a.abdel-aal@azhar.edu.eg
Ahmed
Abd raboh
Biophysics Branch, Physics Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ah_biophysics6@azhar.edu.eg
Khairy
Tohamy
0000-0002-1655-5911
physics department,Faculty of Science, Al-Azhar University, Cairo, Egypt
already_a555@yahoo.com
10.21608/ejbbe.2020.41520.1035
In this study, TEOS - CaCl2 and Mixed TEOS-MAPTMS-CaCl2 were synthesized via sol-gel method and evaluated as a bioactive bone substitute. The hydrolysis and condensation were achieved in an (ethanol/water) solution. The ratio of silane to water to ethanol was 1:3:3. Bioactivity of the samples was premeditated via in-vitro test by inundation in simulated body fluid (SBF) to assess the creation of a hydroxyl apatite (HA) layer on the surface of the samples. Structural characterization of the prepared samples was done using Thermal gravimetric analysis (TGA), X-ray diffraction (XRD) and fourier transform infrared (FTIR) spectroscopy. Elemental Analysis was performed using a UV-Vis Spectrophotometer. Results showed the creation of a layer of hydroxyl apatite (HA) on the surface of the samples. It was found that, sample contain hydrocarbon chain (sample doped with MAPTMS) need higher temperature to lose water and ethanol, i.e. organic precursor increases the thermal stability of the samples. In addition a layer of HA were deposited on the surfaces of the two samples after soaking in SBF for 33 days.
biomaterials,SBF,Hybrid,sol-gel,Hydroxyapatite
https://ejbbe.journals.ekb.eg/article_118526.html
https://ejbbe.journals.ekb.eg/article_118526_fa3e1215b0c5b8f3092e010a1fe1c80c.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Effect of Chemotherapy on Liver Metabolism as Measured by PET/CT Scan
75
85
EN
shaimaa
Abdullah
0000-0003-1855-5461
7st. Salah Asar, Shoubra
shaimaaabdullah142@gmail.com
AIDA
Salama
Biophysics Branch, Physics Department, Faculty of Science (Girls branch), Al-Azhar University, Cairo, Egypt
Mohamed
Mohamed Houseni
Radiology Department, National Liver Institute, Menoufia University, Egypt
Asmaa A A
Elsheshiny
Biophysics Branch, Physics Department, Faculty of Science (Girls branch), Al-Azhar University, Cairo, Egypt
10.21608/ejbbe.2020.38085.1033
Purpose: investigate the metabolic changes on liver caused by chemotherapy by utilizing F18-FDG PET/CT scan by using Radiomics.<br /> Methods: 83 Patients with lymphoma underwent PET/CT scan before, after treatment and in follow up stage. Hounsfield units (HU) of liver and spleen ,maximum and mean standard uptake value (SUVmax,SUVmean), mean correlation for background, metabolic tumor volume (MTV), total lesion glycolysis (TLG) were elevated. The image undergo Radiomics's analytical system by using (LIFEx v.4) get 4 histogram -intensity features and textural features find by six gray levels co-occurrence matrix (GLCM) features, all were elevated. <br /> Results: 45 patients (54.2%) during treatment had liver morphological changes in HU of liver, HU of spleen from non contrast CT, HU of liver in CT scan with contrast and liver volume. There are functional changes in SUVmax, MTV, and TLG. Changes in MTV and TLG were related to changes in SUVmax and SUVmean. There are also morphological changes proved by first order statics [histogram features (skewness, entropy, and kurtosis)]. By increasing number of cycles of chemotherapy lead to textural changes proved by second order statics [GLCM features (homogeneity, energy, contrast, correlation, entropy and dissimilarity)] were elevated. <br /> Conclusion: most of features from F18-FDG PET/CT provide strong independent predictors for fatty liver changes in patients with lymphoma under chemotherapy. The utility of such features should be confirmed by larger clinical studies before considering their potential integration into decisional algorithms aimed at personalized medicine.
Chemotherapy,PET/CT scan,Radiomics Software,drugs side effects
https://ejbbe.journals.ekb.eg/article_109436.html
https://ejbbe.journals.ekb.eg/article_109436_e339f60c0a1cab7620d02a733fc85bbe.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology
Egyptian Journal of Biomedical Engineering and Biophysics
1110-8525
2357-0911
21
1
2020
12
01
Low level laser therapy for hyperoxia-induced retinal protein deformations evaluated by FTIR study
87
96
EN
Maha
Sabry
0000-0001-7016-2046
physics department, faculty of science, Al-Azhar university(Girls), Egypt
mahasabry.519@azhar.edu.eg
Salwa
Abdelkawi
Biophysics and Laser Science Unit, Research Institute of Ophthalmology, Giza,
saelkawi@yahoo.com
Aziza
El Saeid
Physics department, Faculty of Science, AL Azhar university (Girls), Cairo, Egypt.
azelsaeid@azhar.edu.eg
Ahlam
El Rashedi
Physics department, Faculty of Science, AL Azhar university (Girls), Cairo, Egypt
ahlammohai@yahoo.com
10.21608/ejbbe.2020.41564.1036
The aim of the present study was to consider the hazardous effects of exposure to high concentrations of O2 (hyperoxia) on retinal protein and evaluate the protective role of low level laser therapy. Fifty Wistar albino rats were divided into three groups as follows: (a) control group (n=10 rats) without neither hyperoxia exposure nor laser treatment, (b) hyperoxia group (n=20 rats) received daily high concentration of O2 (90%), for one week (n=10 rats) and two weeks (n=10 rats) (c) hyperoxia group (n=20 rats) as the previous group was treated with a 670 nm light emitted diode laser two sessions aweek. experimental animals were sacrificed after one week and two weeks (n= 10 eyes rats each), Fourier transform infrared spectrum analysis was applied. <br /> The results confirmed that retinal protein analysis revealed structurel deformation that appeared after hyperoxia in NH–OH and fingerprint regions. strO–Hsym band shifted from 3265±1 cm-1 to 3284±1 cm-1 (p < 0.01) and O-Hstr band width was increased from 118±1 to 150±2 (p < 0.01). CH3 deformation and COOassy bands are mostly affected in fingerprint region. Wavenumber of the first one was moved from 1294 ±2 cm-1 to 1327±1cm-1 (p < 0.01) where the second band was missed. After treatment with LED laser, amide frequency may be enhanced in one week and two weeks with hyperoxia groups. Bands of amide , COOsym, CH3 Deformation and PO2 asymmetric have non-significant change in their frequencies (p˃0.05), Using low level laser therapy showed a clear improvement in retinal protein structure which illustrated by Fourier transform infrared analysis.
Hyperoxia,FTIR Spectroscopy,Low levle laser,Retinal protein
https://ejbbe.journals.ekb.eg/article_118527.html
https://ejbbe.journals.ekb.eg/article_118527_90688c849dc6c4c934a0e8ec36c0ffc3.pdf